The amine oxidases of human placenta and pregnancy plasma.

نویسندگان

  • W G Bradsley
  • M J Crabbe
  • I V Scott
چکیده

1. The purification of monoamine oxidase and diamine oxidase from normal human term placental tissue is described. 2. The properties of these enzymes are reported and compared with the properties of unpurified human pregnancy plasma. 3. This comparison shows that the amine oxidase of pregnancy plasma has properties corresponding to purified placental diamine oxidase, suggesting a placental origin for the plasma enzyme system. 4. Detailed kinetic study of the purified placental diamine oxidase suggests that it has a Ping Pong sequence, a mechanism of action and rate-limiting step similar to the diamine oxidase of pig kidney. 5. It is suggested that the enzyme system is important in protecting the foeto-placental unit from excesses of biogenic amines.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Competitive inhibition of copper amine oxidases by vitamin B hydrochloride in chickpea

Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of th...

متن کامل

Inhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine

Copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to mol...

متن کامل

P-27: The Effect of Oral Morphine Consumptionon Plasma Corticosteron Density and PlacentaDevelopment in Pregnant Wistar Rats

Background: Previous studies have shown that morphine consumption during pregnancy may delay embryo development or cause abnormal nervous system function .The most attention of research focuses on the embryo, whereas it was not attended to the placenta as an important organ which is affected by opioides. The present study focused on the effect of maternal morphine consumption on development of ...

متن کامل

Human Plasma Monoamine Oxidase

Although this human plasma enzyme was shown (1) to have certain characteristics in common with plasma amine oxidases from other mammals (2-6), it could be distinguished from these other plasma enzymes by its substrate specificity. During studies relating to the substrate specificity of the human enzyme, the oxidation of aliphatic amines was noted to have a number of distinctive kinetic features...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Biochemical journal

دوره 139 1  شماره 

صفحات  -

تاریخ انتشار 1974